Open AccessSustained glycolytic oscillations – no need for cyanide
Author(s) -
Poulsen Allan K,
Lauritsen Frants R,
Folke Olsen Lars
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09656.x
Subject(s) - yeast , acetaldehyde , saccharomyces cerevisiae , cyanide , chemistry , biophysics , biochemistry , extracellular , cuvette , fluorescence spectroscopy , chromatography , analytical chemistry (journal) , fluorescence , biology , organic chemistry , physics , quantum mechanics , ethanol
Using fluorescence spectroscopy we detected long trains of macroscopic oscillations in the glycolytic pathway, in whole cell suspensions of Saccharomyces cerevisiae , without addition of cyanide. Such oscillations may be induced if argon or another inert gas is bubbled through the yeast cell suspension. This supports that the synchronizing agent is a volatile compound secreted by the yeast cells, e.g. CO 2 and/or acetaldehyde. Our results show that the rate of acetaldehyde removal is not a crucial parameter to the synchronization of the yeast cells. The sample cell was connected to a membrane inlet mass spectrometer (MIMS) for online determination of extracellular non‐polar compounds. Oscillations in the secretion of CO 2 were detected using the MIMS.