Open AccessA hydrogen‐evolving enzyme is present in Frankia sp. R43
Author(s) -
Mohapatra Anasuya,
Leul Melakeselam,
Mattsson Ulrika,
Sellstedt Anita
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09652.x
Subject(s) - frankia , actinorhizal plant , biology , hydrogenase , nitrogenase , microbiology and biotechnology , nitrogen fixation , ralstonia , enzyme , biochemistry , root nodule , bacteria , genetics
The ability to evolve hydrogen using methyl viologen as an electron donor was assayed in the nitrogen‐fixing actinomycetes Frankia sp. R43 and Frankia sp. KB5. To further examine the nature of hydrogen‐evolving enzymes that may be present in these organisms immunological studies were performed. Under anaerobic conditions (both nitrogen‐limiting and nitrogen‐containing) Frankia sp. R43 but not Frankia sp. KB5 evolved hydrogen, which was not linked to NAD‐reducing activity. Immunological analysis of total protein from Frankia sp. R43 and Frankia sp. KB5 using an antiserum raised against Ralstonia eutropha HoxF, recognized an antigen in Frankia sp. R43 but not in Frankia sp. KB5. Immunogold labeling using antibodies raised against the R. eutropha HoxH recognized sites in both hyphae and vesicles of Frankia sp. R43, but not in Frankia sp. KB5. Based on these physiological and immunological findings, we conclude that Frankia sp. R43 has a hydrogen‐evolving hydrogenase.