Open AccessMethylthioacetaldehyde, a possible intermediate metabolite for the production of volatile sulphur compounds from l ‐methionine by Lactococcus lactis
Author(s) -
Bonnarme Pascal,
Amarita Felix,
Chambellon Emilie,
Semon Etienne,
Spinnler Henry E,
Yvon Mireille
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09631.x
Subject(s) - methanethiol , transamination , lactococcus lactis , methionine , sulfur , chemistry , biochemistry , metabolite , organic chemistry , bacteria , biology , amino acid , lactic acid , genetics
Volatile sulphur compounds (VSCs) production from l ‐methionine was studied in Lactococcus lactis . In vitro studies with radiolabelled l ‐methionine and resting cells of L. lactis revealed that l ‐methionine was initially converted to α‐keto‐γ‐methylthiobutyrate (KMBA) by a transamination reaction. A part of KMBA was subsequently chemically converted to methylthioacetaldehyde, methanethiol and dimethylsulphides. Chemical conversion of KMBA to methylthioacetaldehyde was dependent on pH, Mn(II) and oxygen. Since methanethiol and dimethylsulphide production was highly related to that of methylthioacetaldehyde, the latter compound was proposed as being an intermediate in VSCs production by L. lactis.