Open AccessThe 3′ termini of small RNAs in Trypanosoma brucei
Author(s) -
Hitchcock Robert A,
Zeiner Gusti M,
Sturm Nancy R,
Campbell David A
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09629.x
Subject(s) - trypanosoma brucei , trypanosoma , biology , computational biology , genetics , gene
Post‐transcriptional 3′ end formation is an essential step in the maturation of most small RNAs. Knowledge of the precise 3′ ends of mature RNAs is essential for defining 3′‐processing activities. We have mapped the mature 3′ ends of Spliced Leader RNA, the U1, U2, U4, U5, and U6 small nuclear RNAs, the U3 small nucleolar RNA, and the 5S and 5.8S ribosomal RNAs by ligation‐mediated PCR in Trypanosoma brucei . With the exception of U5, two classes of 3′ ends were observed: flush with the base of a stem–loop structure, and 1–2 nt extended from a stem–loop. Multiple mechanisms and structural features are likely to influence 3′ end maturation of RNAs.