Open AccessThe light‐responsive promoter of cyanobacterial ORF76 gene overlaps with the htpG terminator
Author(s) -
Plansangkate Prae,
Faaroonsawat Sirirat,
Panyim Sakol,
Chungjatupornchai Wipa
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09608.x
Subject(s) - terminator (solar) , gene , synechococcus , promoter , biology , genetics , transcription (linguistics) , coding region , gene expression , cyanobacteria , bacteria , ionosphere , linguistics , physics , philosophy , astronomy
A Synechococcus PCC7942 gene, encoding 76 amino acids of an unknown protein (designated ORF76), is located at the same orientation and downstream of the htpG gene. The processed site of ORF76 transcripts is located at position +39 with respect to the true transcription initiation site. The non‐ E. coli σ 70 ‐like basal promoter of ORF76 (−51 to −9) is controlled by three cis ‐acting elements: positively acting element (−160 to −86), negative regulatory element (−86 to −51) and light‐responsive element (−51 to +63) that, together, respond to high light induction at transcriptional level. The ORF76 gene is expressed as monocistronic transcript. The promoter elements of ORF76 gene overlap with the coding sequence and 3′ end formation signal of htpG gene.