Open AccessSynthesis and immunogenicity of polysaccharide–protein conjugate composed of galactoglucoxylomannan of Cryptococcus laurentii
Author(s) -
Bystrický Slavomír,
Paulovicová Ema,
Machová Eva
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09604.x
Subject(s) - immunogen , immunogenicity , conjugate , conjugate vaccine , antigen , immune system , isotype , antibody , microbiology and biotechnology , conjugated system , biology , booster dose , chemistry , immunology , immunization , monoclonal antibody , mathematical analysis , mathematics , organic chemistry , polymer
Galactoglucoxylomannan (GalGXMan) antigen of Cryptococcus laurentii was conjugated to protein carrier by a simple one step reaction. The conjugate was immunogenic in rabbits and reinjection elicited booster response with significant increase of serum IgG (H + L) level. Induction of this Ig‐isotype was confirmed in experiments with Protein A. Effectiveness of immune serum to inhibit the growth of C. laurentii was demonstrated. The results indicate that the prepared conjugate could be considered as effective immunogen with potential for incorporation in the vaccine.