Open AccessMolecular typing and phylogenetic analysis of enteroinvasive Escherichia coli using the fli C gene sequence
Author(s) -
Amhaz Juliana M.K,
Andrade Agda,
Bando Silvia Y,
Tanaka Tânia L,
MoreiraFilho Carlos A,
Martinez Marina B
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09597.x
Subject(s) - biology , phylogenetic tree , flagellin , genetics , escherichia coli , gene , microbiology and biotechnology , typing , amplicon , polymerase chain reaction
Non‐motile enteroinvasive Escherichia coli (EIEC) is serotyped based only on O antigen polymorphism, since H antigen epitopes, present on the flagellins, cannot be characterised in these bacteria. In this study, we demonstrate the presence of the flagellin‐coding fli C gene in non‐motile EIEC strains. Moreover, we were able to group the 11 most common non‐motile EIEC serotypes into six different RFLP patterns of the fli C gene. Amplicons representing each RFLP pattern were sequenced. Sequencing data were used to construct a phylogenetic tree which showed two main clusters: one sharing similarity with Shigella dysenteriae and pathogenic E. coli , and the other being closer to non‐pathogenic E. coli .