Open AccessDesign of 16S rRNA‐targeted oligonucleotide probes and microbial community analysis in the denitrification process of a saline industrial wastewater treatment system
Author(s) -
Yoshie Sachiko,
Noda Naohiro,
Tsuneda Satoshi,
Hirata Akira,
Inamori Yuhei
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09585.x
Subject(s) - halomonas , denitrification , 16s ribosomal rna , microbiology and biotechnology , biology , wastewater , sewage treatment , microbial population biology , oligomer restriction , bacteria , environmental chemistry , chemistry , oligonucleotide , nitrogen , environmental science , environmental engineering , biochemistry , gene , genetics , organic chemistry
Three 16S rRNA‐targeted oligonucleotide probes, namely, PSMg437 targeting several members of the genus Pseudomonas , Hlm474 targeting several members of the genus Halomonas , and Clw844 targeting several members of the genus Colwellia , were designed. The microbial community structure and nitrogen removal ability of nitrate‐containing saline wastewater treatment systems with anaerobic packed bed and fluidized bed were monitored. Direct cell counting using fluorescence in situ hybridization (FISH) images revealed that various phylogenetic groups were evenly distributed in the anaerobic packed bed whereas members of the genus Halomonas were dominant particularly in the anaerobic fluidized bed. These results suggest that the microbial communities produced by different flow conditions correlated with denitrification ability in saline industrial wastewater treatment systems.