Open AccessAcquired resistance to bile increases fructose‐6‐phosphate phosphoketolase activity in Bifidobacterium
Author(s) -
Sánchez Borja,
Noriega Luis,
RuasMadiedo Patricia,
ReyesGavilán Clara G.,
Margolles Abelardo
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09564.x
Subject(s) - bifidobacterium bifidum , fructose , bifidobacterium , biochemistry , catabolism , enzyme , chemistry , strain (injury) , carbohydrate , biology , lactobacillus , fermentation , anatomy
Two Bifidobacterium strains with acquired resistance to bile were used in this study. Significant differences on membrane‐associated protein profiles were found between the bile resistant derivatives and their corresponding original strains. One of the major species detected in one of the resistant derivatives had an apparent denatured molecular mass of approximately 90 kDa, and was identified as xylulose‐5‐phosphate/fructose‐6‐phosphate phosphoketolase, the key enzyme of Bifidobacterium carbohydrate catabolism. Phosphoketolase activity was considerably higher in membrane preparations and cell‐free extracts of the two bile resistant derivatives. This correlated to a greater consumption rate of glucose in resistant strains. Fructose‐6‐phosphate phosphoketolase activity in the strain Bifidobacterium bifidum CECT4549 and its resistant derivative was found to be partially associated with the cytoplasmic membrane through weak interactions.