Open AccessAn improved method for deleting large regions of Escherichia coli K‐12 chromosome using a combination of Cre/ loxP and λ Red
Author(s) -
Fukiya Satoru,
Mizoguchi Hiroshi,
Mori Hideo
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09551.x
Subject(s) - cre lox recombination , genome , escherichia coli , biology , chromosome , genetics , comparative genomic hybridization , recombination , microbiology and biotechnology , gene , transgene , genetically modified mouse
We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, λ Red and Cre/ loxP . The loxP site could be rapidly and efficiently integrated in the genome by λ Red and large deletions of both 117‐ and 165‐kbp regions could be generated in 100% efficiency by Cre/ loxP . Comparative genomic hybridization microarray experiments of deletion strains indicated that deletions were generated only in expected regions of the genome. These results have demonstrated that the method is useful for genome engineering in E. coli .