Open AccessIdentification and genetic characterisation of orthopaedic Staphylococcus isolates collected in Italy by automated Eco RI ribotyping
Author(s) -
Andollina Agnese,
Cesare Alessandra,
Bertoni Giorgia,
Modelli Liliana,
Manfreda Gerardo
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09544.x
Subject(s) - ribotyping , ecori , biology , microbiology and biotechnology , identification (biology) , genetics , genotype , restriction enzyme , gene , botany
The aim of this study was to evaluate the possibility to use automated Eco RI ribotyping to address, during the same analysis, both identification and genetic characterisation of 38 Staphylococcus aureus and 64 coagulase‐negative staphylococci collected from surgical injuries. The ribotyping identification results confirmed those obtained using the API Staph® system for 96% of the isolates. All strains were successfully genotyped and the ribotyping discriminatory power, calculated using the Simpson's index of discrimination, was very high for both groups of staphylococci tested. The same, as well as different biotypes, were identified among isolates with the identical ribotyping profile.