Open AccessDiversity of genetic environment of bla CTX‐M genes
Author(s) -
Lartigue MarieFrédérique,
Poirel Laurent,
Nordmann Patrice
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09534.x
Subject(s) - gene , transposition (logic) , biology , sequence (biology) , insertion sequence , genetics , mobile genetic elements , microbiology and biotechnology , plasmid , genome , transposable element , philosophy , linguistics
Twenty‐four non‐clonally related enterobacterial isolates producing the emerging CTX‐M‐type extended‐spectrum β‐lactamases were recovered from several countries including France, India, Poland, and Turkey. They had been isolated from 2000 to 2003. β‐Lactamases CTX‐M‐2, CTX‐M‐3, CTX‐M‐10, CTX‐M‐14 and CTX‐M‐15 were identified. Most of the isolates produced β‐lactamase CTX‐M‐15. Insertion sequence IS Ecp1 was found upstream of bla CTX‐M‐3 , bla CTX‐M‐10 , bla CTX‐M‐14 and bla CTX‐M‐15 genes. A sequence similar to the inverted right repeat of IS Ecp1 was identified downstream of bla CTX‐M‐3 , bla CTX‐M‐10 and bla CTX‐M‐15 genes suggesting the mobilization of these β‐lactamase genes by transposition events. In addition, Orf513 was identified upstream of the bla CTX‐M‐2 gene. This work further underlined widespread of bla CTX‐M‐15 gene associated with IS Ecp1 .