Open AccessThe pentose phosphate pathway in Trypanosoma cruzi
Author(s) -
Maugeri Dante A.,
Cazzulo Juan J.
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09522.x
Subject(s) - pentose phosphate pathway , trypanosoma cruzi , biochemistry , enzyme , cytosol , pentose , biology , transaldolase , chemistry , glycolysis , parasite hosting , world wide web , computer science , fermentation
The pentose phosphate pathway has been studied in Trypanosoma cruzi , Clone CL Brener. Functioning of the pathway was demonstrated in epimastigotes by measuring the evolution of 14 CO 2 from [1‐ 14 C] or [6‐ 14 C] d ‐glucose. Glucose consumption through the PPP increased from 9.9% to 20.4% in the presence of methylene blue, which mimics oxidative stress. All the enzymes of the PPP are present in the four major developmental stages of the parasite. Subcellular localisation experiments suggested that the PPP enzymes have a cytosolic component, predominant in most cases, although all of them also seem to have organellar localisation(s).