Open AccessIdentification of a Shiga‐toxin type I variant containing an IS 1203 ‐like element, from Shiga‐toxin producing Escherichia coli O157:H7
Author(s) -
Suzuki Masahiro,
Kondo Fumio,
Ito Yuko,
Matsumoto Masakado,
Hata Mami,
Oka Hisao,
Takahashi Masao,
Sakae Kenji
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09513.x
Subject(s) - shiga toxin , escherichia coli , vero cell , toxin , biology , microbiology and biotechnology , gene , shiga like toxin , enterobacteriaceae , genetics , cell culture
We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx 1 gene with an IS 1203 ‐like element (designated as IS 1203 v 1 ). The IS 1203 v 1 was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser–Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS 1203 v 1 hardly affected the stx 1 gene in either its expression or the toxicity of its product.