Open AccessCharacterization of an excreted/secreted antigen form of 14‐3‐3 protein in Toxoplasma gondii tachyzoites
Author(s) -
Assossou Olga,
Besson Françoise,
Rouault JeanPierre,
Persat Florence,
Ferrandiz Josette,
Mayençon Martine,
Peyron François,
Picot Stèphane
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09508.x
Subject(s) - toxoplasma gondii , secretion , cycloheximide , toxoplasmosis , antigen , recombinant dna , secretory protein , antibody , cytosol , biology , microbiology and biotechnology , protein biosynthesis , biochemistry , virology , immunology , gene , enzyme
The 14‐3‐3 protein was shown to be present into the parasitophorous vacuole of Toxoplasma gondii ‐infected human monocyte cells and in the excreted/secreted antigens (ESA). The ESA 14‐3‐3 protein migrates electrophoretically as the cytosol and the main membranous 14‐3‐3 isoforms. The excretion/secretion of 14‐3‐3 was not sensitive to cycloheximide, a protein synthesis inhibitor, even at a concentration which inhibited the production of 14‐3‐3 inside the tachyzoites. Recombinant 14‐3‐3/GST protein was used to test the presence of 14‐3‐3 antibodies in different human sera. A positive immunoreactivity was observed with sera corresponding to acute toxoplasmosis and a possible involvement of 14‐3‐3 in host immunity is discussed.