Open AccessCerato‐platanin protein is located in the cell walls of ascospores, conidia and hyphae of Ceratocystis fimbriata f. sp. platani
Author(s) -
Boddi Silvia,
Comparini Cecilia,
Calamassi Roberto,
Pazzagli Luigia,
Cappugi Gianni,
Scala Aniello
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09501.x
Subject(s) - conidium , hypha , immunogold labelling , mycelium , ceratocystis , biology , cell wall , botany , microbiology and biotechnology , homothallism , ascospore , septoria , spore , ultrastructure , biochemistry , fungus , gene , mating type
Abstract Cerato‐platanin (CP), a protein of about 12.4 kDa from Ceratocystis fimbriata f. sp. platani ( Cfp ), accumulated in the mycelium and was located in the cell walls of Cfp ascospores, hyphae and conidia suggesting that this protein had a role in forming the fungal cell wall apart from the already known fact that it is secreted early in culture and elicits phytoalexin synthesis and/or plant cell death. The finding was obtained with three immunological techniques: a quantitative ELISA which determines the amount of CP in the mycelium, an immunofluorescence assay, and immunogold labelling to define the exact localization of CP in the Cfp cells.