Open AccessLignin peroxidase is involved in the biobleaching of manganese‐less oxygen‐delignified hardwood kraft pulp by white‐rot fungi in the solid‐fermentation system
Author(s) -
Machii Yoshiharu,
Hirai Hirofumi,
Nishida Tomoaki
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09493.x
Subject(s) - phanerochaete , manganese peroxidase , chrysosporium , kraft process , lignin peroxidase , chemistry , hardwood , laccase , fermentation , lignin , pulp (tooth) , food science , kraft paper , white rot , botany , pulp and paper industry , microbiology and biotechnology , enzyme , biochemistry , biology , organic chemistry , medicine , pathology , engineering
Biobleaching of manganese‐less oxygen‐delignified hardwood kraft pulp (E‐OKP) by the white‐rot fungi Phanerochaete sordida YK‐624 and P. chrysosporium was examined in the solid‐state fermentation system. P. sordida YK‐624 possessed a higher brightening activity than P. chrysosporium , increasing pulp brightness by 13.4 points after seven days of treatment. In these fermentation systems, lignin peroxidase (LiP) activity was detected as the principle ligninolytic enzyme, and manganese peroxidase and laccase activities were scarcely detected over the course of treatment of E‐OKP by either fungus. Moreover, a linear relationship between brightness increase and cumulative LiP activity was observed under all tested culture conditions with P. sordida YK‐624 and P. chrysosporium . These results indicated that LiP is involved in the brightening of E‐OKP by both white‐rot fungi.