Open AccessPectins and pectic‐oligosaccharides inhibit Escherichia coli O157:H7 Shiga toxin as directed towards the human colonic cell line HT29
Author(s) -
OlanoMartin Estibaliz,
Williams Mark R,
Gibson Glenn R,
Rastall Robert A
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2003.tb11504.x
Subject(s) - escherichia coli , shiga toxin , toxin , chemistry , oligosaccharide , toxicity , enzyme , hydrolysis , biochemistry , pectin , cell wall , microbiology and biotechnology , biology , organic chemistry , gene
Pectins and pectic‐oligosaccharides, as derived by controlled enzymatic hydrolysis, were evaluated for their ability to interfere with the toxicity of Shiga‐like toxins from Escherichia coli O157:H7. Both types of material resulted in some degree of protection but this was significantly higher ( P >0.01) with the oligosaccharide fractions (giving 90–100% cell survival, compared to 70–80% with the polymer). An effect of methylation on the protective effect was detected with lower degrees being more active. The pectic‐oligosaccharides and galabiose, the minimum toxin receptor analogue, were shown to inhibit toxicity and were both protective at 10 mg ml −1 , but not at lower concentrations.