Open AccessSucrose accumulation in salt‐stressed cells of agp gene deletion‐mutant in cyanobacterium Synechocystis sp. PCC 6803
Author(s) -
Miao Xiaoling,
Wu Qingyu,
Wu Guifang,
Zhao Nanming
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2003.tb11500.x
Subject(s) - synechocystis , mutant , sucrose , biochemistry , biology , osmoprotectant , cyanobacteria , gene , microbiology and biotechnology , chemistry , bacteria , proline , genetics , amino acid
The agp gene encoding the ADP‐glucose pyrophosphorylase is involved in cyanobacterial glycogen synthesis and glucosylglycerol formation. By in vitro DNA recombination technology, a mutant with partial deletion of agp gene in the cyanobacterium Synechocystis sp. PCC 6803 was constructed. This mutant could not synthesize glycogen or the osmoprotective substance glucosylglycerol. In the mutant cells grown in the medium containing 0.9 M NaCl for 96 h, no glucosylglycerol was detected and the total amount of sucrose was 29 times of that of in wild‐type cells. Furthermore, the agp deletion mutant could tolerate up to 0.9 M salt concentration. Our results suggest that sucrose might act as a similar potent osmoprotectant as glucosylglycerol in cyanobacterium Synechocystis sp. PCC 6803.