Open AccessMutations in sit B and sit D genes affect manganese‐growth requirements in Sinorhizobium meliloti
Author(s) -
Platero Raúl A,
Jaureguy Melina,
Battistoni Federico J,
Fabiano Elena R
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2003.tb11499.x
Subject(s) - sinorhizobium meliloti , manganese , affect (linguistics) , gene , genetics , biology , chemistry , philosophy , mutant , linguistics , organic chemistry
Two transposon‐induced mutants of Sinorhizobium meliloti 242 were isolated based on their inability to grow on rich medium supplemented with the metal chelator ethylenediamine di‐ o ‐hydroxyphenylacetic acid (EDDHA) and either heme‐compounds or siderophores as iron sources. Tagged loci of these mutants were identified as sitB and sitD genes. These genes encode components of an ABC (ATP‐binding cassette) metal‐type permease in several Gram‐negative bacteria. In this work, the phenotypes of these two mutants were compared with those of two siderophore‐mediated iron transport mutants. The results strongly implicate a role of the sit genes in manganese acquisition when this metal is limiting in S. meliloti .