Open AccessProduction of d ‐(−)‐3‐hydroxyalkanoic acid by recombinant Escherichia coli
Author(s) -
Zhao Kai,
Tian Ge,
Zheng Zhong,
Chen JinChun,
Chen GuoQiang
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2003.tb11498.x
Subject(s) - acetic acid , fermentation , acrylic acid , escherichia coli , chemistry , nuclear chemistry , overproduction , biochemistry , extracellular , enzyme , organic chemistry , copolymer , polymer , gene
Pathways for extracellular production of chiral d ‐(−)‐3‐hydroxybutyric acid (3HB) and d ‐(−)‐3‐hydroxyalkanoic acid (mcl‐3HA) were constructed by co‐expression of genes of β‐ketothiolase ( phbA ), acetoacetyl‐CoA reductase ( phbB ) and 3‐hydroxyacyl‐ACP CoA transacylase ( phaG ), respectively, in Escherichia coli strain DH5α. The effect of acrylic acid and glucose on production of both 3HB and mcl‐3HA was investigated. It was found that the addition of acrylic acid significantly increased production of 3HB and mcl‐3HA consisting of 3‐hydroxyoctanoic acid and 3‐hydroxydecanoic acid in a ratio of 1:3 from 199 mg l −1 to 661 mg l −1 and from 27 mg l −1 to 135 mg l −1 , respectively, in shake flask studies when glucose was present in the medium at the very beginning of fermentation. The timing of glucose addition had no effect on 3HB production. In contrast, mcl‐3HA production was affected by glucose addition, an mcl‐3HA concentration of 193 mg l −1 was obtained when glucose was added to the culture at 12 h. A more than seven‐fold increase was obtained when compared with that in medium containing glucose at the beginning of fermentation. However, a decrease in production of 3HB and mcl‐3HA was found when glucose was added at 12 h to the culture containing acrylic acid. The repressive effect of acrylic acid on acetic acid production was also evaluated and discussed.