Open AccessThe enterotoxin T (BcET) from Bacillus cereus can probably not contribute to food poisoning
Author(s) -
Choma Caroline,
Granum Per Einar
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11464.x
Subject(s) - bacillus cereus , enterotoxin , vero cell , microbiology and biotechnology , cereus , escherichia coli , clone (java method) , monoclonal antibody , biology , antibody , chemistry , bacteria , cell culture , biochemistry , gene , genetics
A polymerase chain reaction (PCR) fragment from strain NVH 38 (containing bceT ) was cloned, sequenced and expressed in Escherichia coli . This sequence showed 50–60% identity to the original. When this bceT clone was expressed in E. coli no biological activity was found in either supernatants or cell extracts. Cell extracts from the Bacillus cereus strains (NVH 38 and B‐4ac) were also negative on Vero cells. Neutralisation of supernatant from B. cereus B‐4ac using a monoclonal antibody (reacting with NheB and HblD) abolished the activity. A test for cytotoxic enterotoxins was negative for both cell extracts and supernatants. Our data suggest that BcET either has an unknown type enterotoxic action or non at all.