Open AccessTranscription of sterol Δ 5,6 ‐desaturase and sterol 14α‐demethylase is induced in the plant pathogenic ascomycete, Leptosphaeria maculans , during treatment with a triazole fungicide
Author(s) -
Griffiths Katherine M,
Howlett Barbara J
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11459.x
Subject(s) - ergosterol , sterol , demethylase , biology , leptosphaeria maculans , biochemistry , triazole , fungicide , gene , chemistry , botany , cholesterol , epigenetics , organic chemistry
Two genes whose derived amino acid sequences closely resemble the ergosterol biosynthetic enzymes, sterol Δ 5,6 ‐desaturase ( erg3 ) and sterol 14α‐demethylase ( erg11 ), were cloned from the plant pathogenic fungus Leptosphaeria maculans . Transcript levels of both these genes increased following exposure of L. maculans to the triazole fungicide, flutriafol, which specifically inhibits the ergosterol biosynthetic pathway. This induction may be due to a decrease in ergosterol content or to abnormal levels of the ergosterol precursor, 24‐methylene dihydrolanosterol.