Open AccessCharacterization of outer membrane efflux proteins OpmE, OpmD and OpmB of Pseudomonas aeruginosa : molecular cloning and development of specific antisera
Author(s) -
Murata Takeshi,
Gotoh Naomasa,
Nishino Takeshi
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11456.x
Subject(s) - efflux , pseudomonas aeruginosa , antiserum , biology , gene , operon , gene product , multiple drug resistance , bacterial outer membrane , microbiology and biotechnology , gene expression , drug resistance , biochemistry , escherichia coli , bacteria , genetics , antibody
The third genes, opmE , opmD and opmB , of multidrug efflux operons deduced from the Pseudomonas aeruginosa PAO1 genome data were cloned by polymerase chain reaction. The opmB gene product showed functional cooperation with inner membrane‐associated components, MexAB, MexCD and MexXY, of the previously characterized multidrug efflux systems responsible for resistance to antimicrobial agents and extrusion of ethidium. The opmE and opmD gene products did not show functional cooperation. Immunoblots using a specific rabbit antiserum demonstrated, through exponential to stationary phases, constant expression of opmB and growth phase‐dependent expression of opmD .