Open AccessCharacterization of the GO system of Pseudomonas aeruginosa
Author(s) -
Oliver Antonio,
Sánchez Juan Manuel,
Blázquez Jesús
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11452.x
Subject(s) - complementation , pseudomonas aeruginosa , gene , escherichia coli , plasmid , biology , sequence analysis , gene product , mutation , genetics , cloning (programming) , operon , microbiology and biotechnology , phenotype , bacteria , gene expression , computer science , programming language
The mutT , mutM , and mutY genes of the GO system of the Pseudomonas aeruginosa PAO1 strain have been characterized by cloning, sequencing, and complementation analysis. The three genes, when cloned in a plasmid, were able to complement the high mutation frequency of the corresponding Escherichia coli deficient strains. Our results demonstrate that the putative mutT , mutM , and mutY gene products from P. aeruginosa are able to perform the expected activity. In addition, the sequence of the P. aeruginosa mutT gene strongly suggested that the product of this gene has a bifunctional activity in P. aeruginosa , being the C‐terminal part 40% identical to a consensus sequence of thiamine monophosphate synthases. Our results also demonstrated that the N‐terminal part of the protein is necessary and sufficient for the 8‐oxodGTP hydrolase activity.