Open AccessCellular localisation of the clamp protein during DNA replication
Author(s) -
Kongsuwan Kritaya,
Dalrymple Brian P,
Wijffels Gene,
Jennings Phillip A
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11444.x
Subject(s) - green fluorescent protein , replication factor c , dna replication , control of chromosome duplication , biology , microbiology and biotechnology , origin recognition complex , dna , dna replication factor cdt1 , replication protein a , dna clamp , eukaryotic dna replication , gene , genetics , dna binding protein , polymerase chain reaction , reverse transcriptase , transcription factor
The β subunit of Escherichia coli DNA polymerase III holoenzyme was fused to the green fluorescent protein GFP. The gene fusion under the control of the heterologous lac promoter was used to replace the wild‐type allele in the chromosome. The formation of GFP‐β fluorescent foci in GFP‐β expressing cells required DNA replication and their number per cell was dependent on cell growth. Examination of GFP‐β foci in a synchronous round of replication suggested that DNA replication was accompanied by the recruitment of GFP‐β foci near the midcell, followed by the rapid migration of the foci in opposite directions to the 1/4 and 3/4 positions during DNA replication.