Open AccessHeterologous expression of nonribosomal peptide synthetases in B. subtilis : construction of a bi‐functional B. subtilis / E. coli shuttle vector system
Author(s) -
Doekel Sascha,
Eppelmann Katrin,
Marahiel Mohamed A
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11434.x
Subject(s) - nonribosomal peptide , bacillus subtilis , shuttle vector , heterologous , heterologous expression , escherichia coli , biology , peptide , biochemistry , gene , biosynthesis , expression vector , bacteria , vector (molecular biology) , recombinant dna , genetics
A major obstacle in investigating the biosynthesis of pharmacologically important peptide antibiotics is the heterologous expression of the giant biosynthetic genes. Recently, the genetically engineered strain Bacillus subtilis KE30 has been reported as an excellent surrogate host for the heterologous expression of an entire nonribosomal peptide synthetase (NRPS) gene cluster. In this study, we expand the applicability of this strain, by the development of four Escherichia coli / B. subtilis shuttle expression vectors. Comparative overproduction of hybrid NRPS proteins derived from both organisms revealed a significant beneficial effect of overproducing proteins in B. subtilis KE30 as underlined by the production of stable nondegradative proteins, as well as the formation of active phosphopantetheinylated holo‐proteins.