Open AccessSUT1 suppresses sec14–1 through upregulation of CSR1 in Saccharomyces cerevisiae
Author(s) -
Régnacq Matthieu,
Ferreira Thierry,
Puard Julien,
Bergès Thierry
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11431.x
Subject(s) - downregulation and upregulation , mutant , saccharomyces cerevisiae , phenotype , gene , gene expression , biology , biochemistry , genetics
SUT1 constitutive expression in aerobiosis suppressed the ts phenotype of the sec14–1 mutation, restored growth of the sec14 ‐null mutant and corrected the translocation defect of the vacuolar carboxypeptidase Y. Therefore SUT1 was shown to be a novel potent sec14–1 suppressor. Further, the hypoxic gene CSR1 (YLR380W), a Sec14 homolog, was upregulated upon SUT1 constitutive expression. In addition, SUT1 effects on both sec14–1 suppression and on free sterol composition were abolished in a csr1 ‐null background, showing that this gene acts downstream of SUT1 .