Open AccessChange of cell wall chitin content in amphotericin B resistant Kluyveromyces strains
Author(s) -
Bahmed Karim,
Bonaly Roger,
Wathier Michel,
Pucci Bernard,
Coulon Joël
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11421.x
Subject(s) - chitinase , kluyveromyces lactis , chitin , kluyveromyces , cell wall , mutant , chitin synthase , biochemistry , biology , microbiology and biotechnology , polyene , enzyme , chemistry , yeast , saccharomyces cerevisiae , gene , chitosan
The culture of two Kluyveromyces species, Kluyveromyces lactis (ATCC 96897) and Kluyveromyces bulgaricus (ATCC 96631), in the presence of subinhibitory doses of amphotericin B leads to the selection of mutants which are resistant to this polyene. The mutants show an alteration of their cell wall composition with the main change corresponding to an increase of chitin. The enzyme activities involved in the metabolism of this polymer, i.e. chitin synthases and chitinase, were measured. The results demonstrate that in both mutants the activity of chitinase was drastically decreased by 99% in comparison with the activity measured in the corresponding wild‐type strain while no significant change of the chitin synthase I, II and III activities could be detected.