Open AccessEndosomal/lysosomal targeting of a single helper T‐cell epitope of an intracellular bacterium by DNA immunisation induces a specific T‐cell subset and partial protective immunity in vivo
Author(s) -
Uchiyama Hiroshi,
Nagata Toshi,
Yamada Takashi,
Uchijima Masato,
Aoshi Taiki,
Suda Takafumi,
Chida Kingo,
Nakamura Hirotoshi,
Koide Yukio
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11420.x
Subject(s) - epitope , intracellular parasite , biology , endosome , dna vaccination , intracellular , t cell , lysosome , splenocyte , virology , microbiology and biotechnology , immune system , immunization , immunology , antigen , biochemistry , enzyme
We evaluated here the effect of the intracellular targeting of a helper T‐cell (Th) epitope, literiolysin O 215–226 derived from Listeria monocytogenes , on induction of a specific Th by gene gun immunisation. Immunisation of C3H/He mice with pE215LAMP plasmid encoding the Th epitope fused with the endosomal/lysosomal targeting signal of lysosome‐associated membrane protein (LAMP)‐1 gave the epitope‐specific proliferative responses of CD4 + T lymphocytes. In addition, specific interferon‐γ production from the splenocytes was observed. Concomitantly, pE215LAMP‐immunised mice showed moderate, but significant protective immunity against listerial challenge. These results suggest that the intracellular targeting of a Th epitope to endosomal/lysosomal compartments by DNA immunisation is useful for eliciting a specific Th subset in vivo.