Open AccessCompetitive hybridization on spotted microarrays as a tool to conduct comparative genomic analyses of Xylella fastidiosa strains
Author(s) -
Costa de Oliveira Regina,
Yanai Giane M,
Muto Nair H,
Leite Daniela B,
Souza Alessandra A,
Coletta Filho Helvécio D,
Machado Marcos A,
Nunes Luiz R
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11408.x
Subject(s) - xylella fastidiosa , biology , dna microarray , computational biology , comparative genomic hybridization , microarray , genetics , genome , gene , bacteria , gene expression
Xylella fastidiosa strains are responsible for several plant diseases and since such isolates display a broad host range and complex biological behavior, genomic comparisons employing microarray hybridizations may provide an effective method to compare them. Thus, we performed a thorough validation of this type of approach using two recently sequenced strains of this phytopathogen. By matching microarray hybridization results to direct sequence comparisons, we were able to establish precise cutoff ratios for common and exclusive sequences, allowing the identification of exclusive genes involved in important biological traits. This validation will enable the use of microarray‐based comparisons across a wide variety of microorganisms