Open AccessPeptide‐mediated delivery of green fluorescent protein into yeasts and bacteria
Author(s) -
Rajarao Gunaratna Kuttuva,
Nekhotiaeva Natalia,
Good Liam
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11401.x
Subject(s) - bacillus subtilis , escherichia coli , green fluorescent protein , candida albicans , peptide , bacteria , yeast , signal peptide , fluorescence microscope , microbiology and biotechnology , biochemistry , biology , staphylococcus aureus , chemistry , fluorescence , peptide sequence , gene , genetics , physics , quantum mechanics
Stringent microbial cell barriers limit the application of many substances in research and therapeutics. Carrier peptides that penetrate or translocate across cell membranes may help overcome this problem. To assess peptide‐mediated delivery into two yeast and three bacterial species, a range of cell penetrating and signal peptide sequences were fused to green fluorescent protein (GFP), expressed in Escherichia coli , partially purified and incubated with growing cells. Fluorescence microscopy indicated several peptides that mediated delivery. In particular, VLTNENPFSDP efficiently delivered GFP into Candida albicans and Staphylococcus aureus , while YKKSNNPFSD was most efficient for Bacillus subtilis and CFFKDEL for Escherichia coli . Carrier peptides may improve delivery of certain large molecular mass molecules into microorganisms for research and therapeutic applications.