Open AccessDoc‐mediated cell killing in Shigella flexneri using a C1/LacI controlled expression system
Author(s) -
Schofield David A.,
Westwater Caroline,
Dolan Joseph W.,
Norris James S.,
Schmidt Michael G.
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11396.x
Subject(s) - shigella flexneri , lac repressor , repressor , bacteriophage , psychological repression , biology , microbiology and biotechnology , shigella sonnei , lac operon , promoter , chemistry , shigella , gene , gene expression , genetics , escherichia coli
In this report we describe the development of a highly stringent and dually regulated promoter system for Shigella flexneri . Dual regulation was provided by utilizing a promoter susceptible to control by the bacteriophage P1 temperature‐sensitive C1 repressor that in turn was under the transcriptional control of LacI. The level of induction/repression ratios observed was up to 3700‐fold in S. flexneri . The general utility of this promoter system was evaluated by demonstrating that the bacteriophage P1 post‐segregational killer protein Doc mediates a bactericidal effect in S. flexneri . This represents the first report of Doc ( d eath o n c uring)‐mediated killing in this Gram‐negative species.