Open AccessHigh expression of a sucrose non‐fermenting (SNF1)‐related protein kinase from Colletotrichum gloeosporoides f. sp. malvae is associated with penetration of Malva pusilla 1
Author(s) -
Goodwin Paul H,
Chen Grace Y.J
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11387.x
Subject(s) - appressorium , biology , sucrose , colletotrichum , microbiology and biotechnology , fungus , glycerol , botany , gene expression , glycerol kinase , fermentation , penetration (warfare) , gene , biochemistry , operations research , engineering
A sucrose non‐fermenting (SNF1)‐related protein kinase homologue, cgsnf , from Colletotrichum gloeosporoides f. sp. malvae , a hemibiotrophic fungal pathogen of round‐leaved mallow ( Malva pusilla ) was examined. During infection, cgsnf showed a large peak in expression relative to a constitutively expressed fungal actin gene when appressoria had formed during the penetration phase and then showed much lower expression levels during subsequent necrotrophic growth in the host. In pure culture with glucose or glycerol as sole carbon sources, expression levels were similar to that during necrotrophic growth. Expression was consistently higher in glycerol than in glucose cultures, which may reflect a lower cellular energy status in the fungus. These results are consistent with cgsnf having a role in transmitting nutritional signals, which may be involved with host penetration.