Open AccessBan AI a new isoschizomer of the type II restriction endonuclease Hae III discovered in a Bacillus anthracis isolate from Amazon Basin
Author(s) -
Chies Jocelei M,
Dias Ana C. de O.,
Maia Helio M.M,
AstolfiFilho Spartaco
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11376.x
Subject(s) - isoschizomer , restriction enzyme , haeiii , bacillus anthracis , biology , restriction fragment , recognition sequence , microbiology and biotechnology , ecori , dna , genetics , gene , genotype , bacteria , restriction fragment length polymorphism , gene expression , dna methylation
Bacillus anthracis was isolated and identified from a bacterial collection of samples from the Amazon river bank. Type II restriction endonuclease activity was detected in this prokaryote, the enzyme was purified, the molecular mass of the native protein estimated by gel filtration, and optima pH, temperature and salt requirements were determined. Quality control assays showed complete absence of ‘non‐specific nucleases’. Restriction cleavage analysis and DNA sequencing of restriction fragments allowed unequivocal demonstration of 5′‐GG↓CC‐3′ as the recognition sequence. This enzyme was named Ban AI and is therefore an isoschizomer of the prototype restriction endonuclease Hae III. This is the first report of a type II restriction endonuclease identified, purified from a natural isolate of B. anthracis .