Open AccessA proteomic study of Escherichia coli O157:H7 NCTC 12900 cultivated in biofilm or in planktonic growth mode
Author(s) -
Trémoulet Frédéric,
Duché Ophélie,
Namane Abdelkader,
Martinie Brigitte,
Labadie JeanClaude
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11363.x
Subject(s) - biofilm , escherichia coli , microbiology and biotechnology , biology , bacteria , enterobacteriaceae , biochemistry , gene , genetics
Escherichia coli 0157:H7 biofilms were studied by a new method of cultivation in order to identify some of the proteins involved in the biofilm phenotype. A proteomic analysis of sessile or planktonic bacteria of the same age was carried out by two‐dimensional electrophoresis, matrix‐assisted laser desorption ionization‐time of flight mass spectrometry (MALDI‐TOF‐MS) and database searching. Comparison of two‐dimensional gels showed clear differences between protein patterns of sessile and planktonic cells. Fourteen proteins increased in biofilms, whereas three decreased. From these 17 proteins, 10 were identified by MALDI‐TOF‐MS and could be classified into four categories according to their function: (1) general metabolism proteins (malate dehydrogenase, thiamine‐phosphate pyrophosphorylase), (2) sugar and amino acid transporters ( d ‐ribose‐binding periplasmic protein, d ‐galactose‐binding protein, YBEJ), (3) regulator proteins (DNA starvation protein and H‐NS) and (4) three proteins with unknown function. The results of this study showed that E. coli O157:H7 modified the expression of several proteins involved in biofilm growth mode.