Open AccessImpact of feed processing and mixed ruminal culture on the fate of recombinant EPSP synthase and endogenous canola plant DNA
Author(s) -
Alexander Trevor W,
Sharma Ranjana,
Okine Erasmus K,
Dixon Walter T,
Forster Robert J,
Stanford Kim,
McAllister Tim A
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11357.x
Subject(s) - canola , recombinant dna , endogeny , biology , rumen , dna , biochemistry , genetically modified organism , dna shuffling , gene , food science , fermentation , directed evolution , mutant
The impact of feed processing and in vitro ruminal cultures on the persistence of recombinant and canola‐specific endogenous DNA was studied using various canola substrates (whole seed, cracked seed, meal and diet). For both, parental and genetically modified substrates, ribulose‐1,5‐bisphosphate carboxylase/oxygenase gene was amplifiable up to varying time points. Persistence of recombinant DNA, encoding 5‐enolpyruvylshikimate‐3‐phosphate synthase (1363 bp) was detected up to 8 h for meal and 4 h for mixed diet. Upon processing of canola, DNA large enough to contain intact plant genes remains. In an in vitro environment, plant DNA was rapidly degraded upon its release into rumen fluid.