Open AccessThe identification of a tetracycline resistance gene tet (M), on a Tn 916 ‐like transposon, in the Bacillus cereus group
Author(s) -
Agersø Yvonne,
Jensen Lars Bogø,
Givskov Michael,
Roberts Marilyn C
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11355.x
Subject(s) - bacillus cereus , tetracycline , microbiology and biotechnology , transposable element , biology , bacteria , bacillus thuringiensis , cereus , bacillales , bacillaceae , gene , bacillus anthracis , bacillus (shape) , bacillus subtilis , antibiotics , genetics , mutant
In order to investigate whether resistance genes present in bacteria in manure could transfer to indigenous soil bacteria, resistant isolates belonging to the Bacillus cereus group ( Bacillus cereus, Bacillus anthracis and Bacillus thuringiensis ) were isolated from farm soil (72 isolates) and manure (12 isolates) samples. These isolates were screened for tetracycline resistance genes ( tet (K), tet (L), tet (M), tet (O), tet (S) and tet (T)). Of 88 isolates examined, three (3.4%) isolates carried both tet (M) and tet (L) genes, while four (4.5%) isolates carried the tet (L) gene. Eighty‐one (92.1%) isolates did not contain any of the tested genes. All tet (M) positive isolates carried transposon Tn 916 and could transfer this mobile DNA element to other Gram‐positive bacteria.