Open AccessEffect of Vitreoscilla hemoglobin biosynthesis in Escherichia coli on production of poly(β‐hydroxybutyrate) and fermentative parameters
Author(s) -
Yu Huimin,
Shi Yue,
Zhang Yanping,
Yang Shengli,
Shen Zhongyao
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11351.x
Subject(s) - recombinant dna , escherichia coli , strain (injury) , biosynthesis , oxygen , biochemistry , chemistry , gene , biology , organic chemistry , anatomy
In order to attain high cell density and low cost production of poly(β‐hydroxybutyrate) (PHB), the Vitreoscilla globin gene ( vgb ) was introduced into a novel recombinant strain, Escherichia coli VG1 (pTU14). Experiments showed that the expression of vgb was under the regulation of dissolved oxygen (DO) in broth and the introduction of vgb in VG1 (pTU14) induced the parent promotion effect on cell growth and PHB accumulation, especially under low DO conditions. Further experiments indicated that the introduction of vgb in VG1 (pTU14) not only decreased the critical oxygen concentration, but also affected the volumetric oxygen transfer coefficient of the recombinant strain.