Open AccessMetabolic engineering of Escherichia coli for the production of medium‐chain‐length polyhydroxyalkanoates rich in specific monomers
Author(s) -
Park Si Jae,
Park Jong Pil,
Lee Sang Yup
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11350.x
Subject(s) - polyhydroxyalkanoates , escherichia coli , metabolic engineering , chemistry , biochemistry , bacteria , biology , enzyme , genetics , gene
The Escherichia coli fabG Ec gene and the Pseudomonas aeruginosa rhlG Pa gene, which encode 3‐ketoacyl‐acyl carrier protein reductase, were expressed in E. coli W3110 and its fadA mutant strain WA101 to examine their roles in medium‐chain‐length (MCL) polyhydroxyalkanoate (PHA) biosynthesis from fatty acids. When one of these 3‐ketoacyl‐acyl carrier protein reductase genes was co‐expressed with the Pseudomonas sp. 61–3 PHA synthase gene ( phaC2 Ps ) in E. coli W3110, MCL‐PHA composed mainly of 3‐hydroxyoctanoate and 3‐hydroxydecanoate was synthesized from sodium decanoate. When the fabG Ec gene and the phaC2 Ps gene were co‐expressed in the fadA mutant E. coli strain WA101, MCL‐PHA rich in 3‐hydroxydecanoate monomer up to 93 mol% was accumulated from sodium decanoate. This was possible by efficiently redirecting 3‐ketoacyl‐coenzymes A from the β‐oxidation pathway to the PHA biosynthesis pathway without losing two carbon units, the strategy of which can be extended for the production of MCL‐PHAs rich in other specific monomers.