Open AccessDegradation of tetracyanonickelate (II) by Cryptococcus humicolus MCN2
Author(s) -
Kwon Hyouk K,
Woo Seung H,
Park Jong M
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11349.x
Subject(s) - formic acid , strain (injury) , chemistry , formamide , cryptococcus , nuclear chemistry , degradation (telecommunications) , ammonia , yeast extract , yeast , nitrogen , biochemistry , microbiology and biotechnology , organic chemistry , biology , fermentation , telecommunications , computer science , anatomy
A new yeast strain capable of degrading free and metallocyanides was isolated from coke‐plant wastewater. The isolated strain designated MCN2 was identified as Cryptococcus humicolus by 26S rDNA sequencing and phylogenetic analysis. During growth of the isolate with KCN as a sole nitrogen source, formamide and formic acid were found as transient intermediates by [ 13 C]nuclear magnetic resonance analysis and ammonia accumulated as a final product in the culture medium. The strain MCN2 could degrade high concentrations of tetracyanonickelate (II) (K 2 Ni(CN) 4 , TCN) up to 65 mM CN within 60 h when a sufficient amount of glucose was supplied as a carbon source. The maximal degradation rate of TCN was 2.5 mM CN h −1 at the initial concentration of 51 mM CN.