Open AccessPutative in vitro expressed gene fragments unique to Mycobacterium avium subspecies paratuberculosis
Author(s) -
Klitgaard Nielsen Kirstine,
Ahrens Peter
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11347.x
Subject(s) - mycobacterium avium subspecies paratuberculosis , paratuberculosis , biology , suppression subtractive hybridization , mycobacterium , microbiology and biotechnology , mycobacterium avium subsp. paratuberculosis , gene , genome , subspecies , in vitro , southern blot , virology , bacteria , genetics , gene expression , cdna library , paleontology
By a suppression subtractive hybridization based method, nine novel Mycobacterium avium subsp. paratuberculosis ( M. paratuberculosis ) fragments of between 318 and 596 bp have been identified and characterized. Database search revealed little or no similarity with other mycobacteria. The uniqueness and diagnostic potential of seven of these fragments in relation to M. paratuberculosis closest relative Mycobacterium avium subsp. avium ( M. avium ) was confirmed by species‐specific PCR and Southern blot. Furthermore, RT‐PCR indicated that eight of the nine fragments originate from areas of the genome that are expressed in vitro.