Open AccessInduction of carbofuran oxidation to 4‐hydroxycarbofuran by Pseudomonas sp. 50432
Author(s) -
Chaudhry G.Rasul,
Mateen A,
Kaskar B,
Sardessai M,
Bloda M,
Bhatti A.R,
Walia Satish K
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11342.x
Subject(s) - carbofuran , chemistry , benzofuran , enzyme , fragmentation (computing) , pseudomonas , phenol , oxidative enzyme , pesticide , biochemistry , chromatography , stereochemistry , organic chemistry , biology , bacteria , ecology , agronomy , genetics
Pseudomonas sp. 50432 biotransformed a highly toxic pesticide, carbofuran (2,3‐dihydro‐2,2‐dimethylbenzofuran‐7‐yl methylcarbamate) to 7‐phenol (2,3‐dihydro‐2,2‐dimethyl‐7‐hydroxy benzofuran) and several unknown metabolites. One of the unknown metabolites identified by gas chromatography/mass spectroscopy was 4‐hydroxycarbofuran (2,3‐dihydro‐2,2‐dimethyl‐4‐hydroxybenzofuran‐7‐yl methylcarbamate). It had a mass (237) similar to 3‐hydroxycarbofuran and 5‐hydroxycarbofuran but different fragmentation patterns. This is the first report in which an inducible oxidative enzyme, hydroxylase, mediated the conversion of carbofuran to 4‐hydroxycarbofuran. A second constitutively synthesized enzyme hyrolase transformed carbofuran to 7‐phenol.