Open AccessMonocyte chemotactic protein‐1 secretion and expression after Toxoplasma gondii infection in vitro depend on the stage of the parasite
Author(s) -
BrenierPinchart MariePierre,
Vigan Inés,
JouvinMarche Evelyne,
Marche Patrice Noél,
Pelet Elisabeth,
Gross Uwe,
AmbroiseThomas Pierre,
Pelloux Hervé
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11323.x
Subject(s) - toxoplasma gondii , secretion , biology , parasite hosting , monocyte , chemotaxis , microbiology and biotechnology , in vitro , messenger rna , pathogenesis , gene expression , immunology , gene , antibody , genetics , biochemistry , receptor , world wide web , computer science
Infection of human fibroblasts with tachyzoites of RH and Prugniaud strains, two different strains of Toxoplasma gondii , significantly increased monocyte chemotactic protein (MCP)‐1 secretion contrary to what happened with bradyzoites of the cystogenetic strain. Quantification of MCP‐1 mRNA by RT‐PCR showed that this phenomenon is regulated at the transcriptional level. Thus, the stage of parasite can be deciding in MCP‐1 induction since only tachyzoites induced MCP‐1 expression and secretion. MCP‐1 induced by tachyzoites could be involved in cell recruitment, as shown by the quantification of MCP1 ARNm by real‐time PCR (LightCycler, Roche Diagnostics), in the pathogenesis of T. gondii infection.