Open AccessDetection of sialoglycomolecules in five plant trypanosomatids and in an insect phytophagous isolate
Author(s) -
Souza dos Santos André Luis,
Sales Alviano Celuta,
Araújo Soares Rosangela Maria
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11319.x
Subject(s) - biology , sialoglycoproteins , insect , kinetoplastida , flagellate , glycoconjugate , botany , trypanosoma , microbiology and biotechnology , biochemistry , sialic acid , virology , protozoal disease , malaria , immunology
Abstract The sialoglycoprotein profiles of five plant trypanosomatids ( Phytomonas spp.) and of one flagellate ( Herpetomonas sp.) isolated from the salivary gland of a phytophagous insect ( Phthia picta ) were analyzed by Western blotting using three distinct lectins (LFA, SNA and MAA), which recognize specifically sialic acid residues in glycoconjugates. All six flagellates presented at least one polypeptide recognized by the lectins, with the exception of Phytomonas françai , which did not show any reactivity with SNA agglutinin. Phytomonas serpens and P. françai showed the most distinct pattern of sialoglycoproteins. Phytomonas mcgheei , Herpetomonas sp. and the two other Phytomonas spp., isolated from latex, displayed an identical sialomolecule profile. We discuss the possible role of the sialoglycoproteins in the physiology of these trypanosomatids.