Open AccessDevelopment of a bipartite method for Fusarium identification based on cellobiohydrolase‐C: CAPS and Western blot analysis
Author(s) -
Hatsch Didier,
Phalip Vincent,
Jeltsch JeanMarc
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11313.x
Subject(s) - humulus lupulus , fusarium , biology , identification (biology) , microbiology and biotechnology , botany , horticulture , pepper
The identification of 12 Fusarium strains isolated from diseased hops ( Humulus lupulus , L.) was achieved by a strategy based on cellobiohydrolase‐C: cleaved amplified polymorphic sequence analysis targeting the gene and the use of an antibody directed against a peptide of the Fusarium graminearum enzyme. This strategy is shown to be rapid and reliable for all the Fusarium of our collection: F. avenaceum , F. graminearum , F. sambucinum , F. sporotrichioides , F. tricinctum and F. venenatum .