Open AccessEvaluation of methods for molecular typing and identification of members of the genus Brevibacterium and other related species
Author(s) -
Alves Artur,
Santos Orlando,
Henriques Isabel,
Correia António
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11307.x
Subject(s) - ribotyping , biology , brevibacterium , typing , amplified ribosomal dna restriction analysis , genomic dna , microbiology and biotechnology , ribosomal dna , genetics , polymerase chain reaction , bacteria , dna , phylogenetics , gene , microorganism
The genus Brevibacterium includes pleomorphic Gram‐positive bacteria with a high mol% G+C content. Species in the genus are difficult to identify by classical methods. The discriminatory power of DNA‐based methods is assessed. Strains representing the four well established Brevibacterium species, and other related bacteria, were compared by amplified ribosomal DNA restriction analysis (ARDRA), repetitive‐sequence‐based PCR (rep‐PCR) and ribotyping. Fingerprinting by rep‐PCR and ribotyping provided complex genomic profiles with the highest discriminatory potential for molecular typing at the strain level, whereas ARDRA showed differentiation from the genus to the species levels. A high degree of heterogeneity within the genus Brevibacterium is apparent, thus indicating that the taxonomy of the genus should be further studied.