Open AccessMetabolism of diphenylurea by a Marinobacter sp. isolated from a contaminated ephemeral stream bed in the Negev Desert
Author(s) -
Sørensen Sebastian R,
Arbeli Ziv,
Aamand Jens,
Ronen Zeev
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11306.x
Subject(s) - metabolite , ephemeral key , chemistry , halophile , strain (injury) , environmental chemistry , metabolism , aniline , metabolic pathway , contamination , microbial biodegradation , microbiology and biotechnology , chromatography , bacteria , microorganism , biology , biochemistry , ecology , organic chemistry , genetics , anatomy
A moderate halophilic Marinobacter sp. (designated strain DPUZ) able to metabolize 1,3‐diphenylurea (DPU) was isolated from a contaminated ephemeral desert stream bed near an industrial complex in the northern part of the Negev Desert (Israel). Metabolism of DPU was accompanied by a transient accumulation of a metabolite identified as aniline using gas chromatography–mass spectrometry, thus indicating a metabolic pathway involving cleavage of the urea bridge between the phenyl structures. Aniline was further degraded without detection of other metabolites suggesting a complete degradation. Strain DPUZ grows at NaCl concentrations between 0.2 and 2.6 M with an optimum at 0.51 M. It grows at a temperature range between 20 and 40°C with an optimum at 35°C. This is the first study on bacterial metabolism of DPU.