Open AccessCharacterization of the Streptomyces violaceoruber SANK95570 plasmids pSV1 and pSV2
Author(s) -
Spatz Kerstin,
Köhn Henning,
Redenbach Matthias
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11290.x
Subject(s) - plasmid , extrachromosomal dna , streptomyces coelicolor , genetics , restriction map , biology , homology (biology) , streptomyces , inverted repeat , cloning (programming) , gene , streptomycetaceae , actinomycetales , microbiology and biotechnology , genome , bacteria , computer science , programming language
We have analyzed the structure of two extrachromosomal elements of the methylenomycin producing actinomycete Streptomyces violaceoruber SANK95570. The presence of the circular plasmid pSV1 which was supposed to contain the genes for methylenomycin biosynthesis could be verified. Physical mapping of pSV1 revealed a size of 175.35 kb for this plasmid. In addition we generated a restriction map for the 100‐kb linear plasmid pSV2. Cloning and sequencing of the terminal ends of pSV2 indicated the presence of 426‐bp terminal inverted repeats. Both pSV2 termini show significant homology to the chromosome ends of Streptomyces coelicolor A3(2) which is a closely related strain to S. violaceoruber SANK95570.