Open AccessPlasmid profiling and antibiotic resistance of Vibrio strains isolated from cultured penaeid shrimp
Author(s) -
MolinaAja Almudena,
GarcíaGasca Alejandra,
AbreuGrobois Alberto,
BolánMejía Carmen,
Roque Ana,
GomezGil Bruno
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11278.x
Subject(s) - carbenicillin , plasmid , microbiology and biotechnology , antibiotic resistance , biology , ampicillin , antibiotics , escherichia coli , shrimp , amp resistance , gene , genetics , fishery
Resistance to different antibiotics was found in 26 of the 30 strains analyzed, more than 70% of the strains analyzed were resistant to carbenicillin and ampicillin and a significant correlation was found between the resistance to both antibiotics. Plasmids were found in 80% of the strains analyzed, and 11 different plasmid profiles were observed. The most common profile obtained had only a 21.2‐kbp plasmid, a significant correlation was found between the presence of this plasmid and resistance to carbenicillin, although some exceptions could be detected. Plasmids were cured from a cephalothin resistant strain and reintroduced into the plasmid‐free cell and into Escherichia coli DH5α, both strains gained resistance to this antibiotic.